Three-layer decellularized basement membrane shows greater long-term corneal staining reduction in dry eye disease

In a small retrospective series, both single- and three-layer dehydrated, decellularized basement membrane (DBM) allografts improved corneal staining in dry eye disease over 6 months, but the three-layer construct produced a significantly greater and more sustained reduction in corneal staining compared with the single-layer option.

The study included 17 eyes treated with either a single-layer DBM (Biovance SL) or a three-layer DBM (Biovance Ocular 3L). Corneal healing was monitored over a 6-month period.

Both groups experienced statistically significant reductions in corneal fluorescein staining at 3 and 6 months. At the 6-month mark, the three-layer DBM group demonstrated a significantly greater reduction in corneal staining compared with the single-layer group.

According to the authors, the three-layer construct was associated with a larger and more sustained improvement in corneal staining than the single-layer option in patients with DED-related epithelial disease.

Reference
Denton M. Comparison of effectiveness of Biovance single and triple layer decellularized basement membranes for treatment of ocular surface disease: a retrospective study. Clin Optom. 2026;18:1-8. doi:10.2147/OPTO.S571568